Ikhithi yokutsalwa kwe-Nucleic Acid (A02)
Umgaqo wokufumanisa
Emva kokukhulula i-DNA ye-genomic ngokwahlula iiseli nge-lysis buffer, i-bead magnetic inokukhetha ngokukhetha i-genomic DNA kwisampulu.Inani elincinane lobumdaka obufunxwa liso lemagnethi linokususwa ngesithinteli sokuhlamba.Kwi-TE, i-bead yamagnetic inokukhulula i-DNA ye-boundgenome, ifumane i-DNA ye-genome ephezulu.Le ndlela ilula kwaye ikhawuleza kwaye umgangatho we-DNA okhutshiweyo uphezulu, onokuhlangabezana nemfuneko yokufunyanwa kwe-DNA methylation.Ngeli xesha, ikhithi yokutsalwa esekwe kwi-bead yamagnetic inokuhambelana ne-automatic nucleic acid extraction, ukuhlangabezana ne-high-throughput nucleic acid extraction tasks.
Amacandelo aphambili e-reagent
Amacandelo aboniswe kwitheyibhile 1:
Itheyibhile 1 Amacandelo e-Reagent kunye nokuLayisha
| Igama lelungu | Amacandelo aphambili | Ubukhulu (48) | Ubungakanani (200) |
| 1. Isithinteli sokwetyisa A | Tris, SDS | 15.8 mL / ibhotile | 66mL/ibhotile |
| 2. ULysis Buffer L | Guanidinium Isothiocyanate, Tris | 15.8 mL / ibhotile | 66mL/ibhotile |
| 3. Hlamba Isithinteli A | NaCl, Tris | 11 mL / ibhotile | 44 mL / ibhotile |
| 4. Hlamba Isithinteli B | NaCl, Tris | 13 mL / ibhotile | 26.5mL/ibhotile *2 |
| 5. TE | Tris, EDTA | 12 mL / ibhotile | 44 mL / ibhotile |
| 6. Isisombululo seProtease K | UProtease K | 1.1mL / iqhekeza | 4.4mL / iqhekeza |
| 7. Ukunqunyanyiswa kwamaso 2 | Amaso magnetic | 0.5mL / iqhekeza | 2.2mL / iqhekeza |
| 8. Imiyalelo yokukhupha i-nucleic acid reagents | / | Ikopi enye | Ikopi enye |
Izinto ezifunekayo kwi-nucleic acid extraction, kodwa azifakwanga kwikhithi:
1. I-Reagent: I-ethanol e-anhydrous, isopropanol, kunye ne-PBS;
2. Izinto ezisetyenziswayo: 50mL centrifuge tube kunye ne-1.5mL EP tube;
3. Izixhobo: Ukuhlamba amanzi, i-pipettes, i-shelf magnetic, i-centrifuge, i-96-well plate (automatic), i-automatic nucleic acid extraction equipment (automatic).
Ulwazi olusisiseko
Iimfuno zesampulu:
I-1.Ukufumanisa kuya kugqitywa phantsi kokugcinwa kweentsuku ze-7 zokushisa kwe-ambient emva kokuqokelela isampuli yeseli ye-cervical exfoliated (engalungiswanga).
I-2.Ukufumanisa kuya kugqitywa phantsi kokugcinwa kweentsuku ze-30 zokushisa kwe-ambient emva kokuqokelela isampuli yeseli ye-cervical exfoliated (elungisiwe).
3.Ukufumanisa kuya kugqitywa phantsi kokugcinwa kweentsuku ezingama-30 zobushushu be-ambient emva kokuqokelelwa komzekelo womchamo;Ubhaqo luyakugqitywa ngexesha emva kokuqokelelwa kweesampulu zeeseli ezikhulisiwe.
Iinkcukacha zokupaka:200 iipcs / ibhokisi, 48 pcs / ibhokisi.
Iimeko zokugcina:2-30℃
Ixesha lokuqinisekisa:Iinyanga ezili-12
Isixhobo esisebenzayo:I-Tianlong NP968-C isixhobo se-nucleic acid extraction, i-Tiangen TGuide S96 i-nucleic acid extraction instrument, i-GENE DIAN EB-1000 isixhobo sokukhupha i-nucleic acid.
Isatifikethi serekhodi yesixhobo sonyango Inombolo./imfuno yobugcisa yemveliso Inombolo.:HJXB No. 20210100.
Umhla wokuvunywa kunye nohlaziyo lwemiyalelo:Umhla wokuvunywa: ngoNovemba 18, 2021
Ngathi
Njengeshishini lobuchwepheshe obuphezulu obusekwe ngo-2018 ziingcali eziphezulu ze-epigenetic, i-Epiprobe igxile ekuxilongweni kwemolekyuli yomhlaza we-DNA methylation kunye noshishino oluchanekileyo lwe-theranostics.Ngesiseko esinzulu setekhnoloji, sijonge ekukhokeleni ixesha leemveliso ezitsha zokuphelisa umhlaza kwi-bud!
Ngokusekwe kuphando lwexesha elide lweqela eliphambili le-Epiprobe, uphuhliso kunye noguquko kwicandelo le-DNA methylation kunye nezinto ezintsha ezintsha, ezidityaniswe neethagethi ezizodwa ze-DNA methylation zomhlaza, sisebenzisa i-algorithm ekhethekileyo yokudibanisa idatha enkulu kunye netekhnoloji yobukrelekrele. ngokuzimeleyo phuhlisa iteknoloji eyodwa yolwelo lwebiopsy ekhuselwe kwilungelo elilodwa lomenzi wechiza.Ngokuhlalutya inqanaba le-methylation yeendawo ezithile zamaqhekeza e-DNA yasimahla kwisampulu, iintsilelo zeendlela zovavanyo lwemveli kunye nokunciphisa utyando kunye nesampulu yokugqobhoza ziyaphetshwa, ezingaphumeleli nje ukufunyaniswa ngokuchanekileyo komhlaza wokuqala, kodwa zikwavumela ukujongwa kwexesha lokwenyani. yokwenzeka komhlaza kunye namandla ophuhliso.
