I-TAGMe DNA Methylation Detection Kits (qPCR) ye-Endometrial Cancer
IIMPAWU ZEMVELISO
Ukuchaneka
Ukuqinisekiswa kwiisampuli zeklinikhi ze-800 kwizifundo eziphindwe kabini ezingaboniyo zamaziko amaninzi, imveliso inomlinganiselo othile we-82.81% kunye novakalelo lwe-80.65%.
Iluncedo
Iteknoloji yokufumanisa i-Me-qPCR ye-methylation yasekuqaleni ingagqitywa kwinqanaba elinye kwiiyure ze-3 ngaphandle kokuguqulwa kwe-bisulfite.
Kwangoko
Ifunyaniswe kwinqanaba elingaphambi komhlaza.
Ukuzenzekela
Isebenza ngebrashi yomlomo wesibeleko kunye neesampulu zePap smear.
UKUSETYENZISWA OKUHLOSIWEYO
Le kiti isetyenziselwa ukufumanisa umgangatho we-in vitro we-hypermethylation ye-gene PCDHGB7 imizekelo yomlomo wesibeleko.Isiphumo esihle sibonisa ukwanda komngcipheko wezilonda ze-endometrial precancerous kunye nomhlaza, odinga uvavanyo olongezelelweyo lwe-histopathological ye-endometrium.Ngokuchasene noko, iziphumo zovavanyo olubi zibonisa ukuba umngcipheko wezilonda ze-endometrial precancerous kunye nomhlaza uphantsi, kodwa umngcipheko awunakukhutshelwa ngaphandle ngokupheleleyo.Ukuxilongwa kokugqibela kufuneka kusekelwe kwiziphumo zoviwo lwe-histopathological ye-endometrium.I-PCDHGB7 lilungu losapho lweprotocadherin γ gene cluster.I-Protocadherin ifunyenwe ukulawula iinkqubo zebhayoloji ezifana nokwanda kweeseli, umjikelezo weseli, i-apoptosis, ukuhlasela, ukufuduka kunye ne-autophagy yeeseli ze-tumor ngeendlela ezahlukeneyo zokubonisa, kunye nokuthuliswa kofuzo okubangelwa yi-hypermethylation yommandla womgqugquzeli kuhambelana ngokusondeleyo nokwenzeka kunye nophuhliso. zomhlaza ezininzi.Kuye kwaxelwa ukuba i-hypermethylation ye-PCDHGB7 idibaniswa neentlobo ezahlukeneyo ze-tumor, ezifana ne-non-Hodgkin lymphoma, umdlavuza webele, umhlaza womlomo wesibeleko, umhlaza we-endometrial kunye nomhlaza wesinyi.
UMGAQO WOKUFUMANA
Le kit iqulethe i-nucleic acid extraction reagent kunye ne-PCR yokufumanisa i-reagent.I-Nucleic acid itsalwa nge-magnetic-bead-based method.Le kit isekelwe kumgaqo we-fluorescence quantitative PCR method, usebenzisa i-methylation-specific real-time real-time reaction ye-PCR ukuhlalutya i-template ye-DNA, kwaye ngaxeshanye ibone iziza ze-CpG ze-PCDHGB7 gene kunye ne-marker yokulawula umgangatho yangaphakathi ye-reference gene fragments G1 kunye ne-G2.Inqanaba le-methylation ye-PCDHGB7 kwisampulu, okanye ixabiso le-Me, libalwa ngokwe-PCDHGB7 gene methylated DNA amplification Ct value kunye nexabiso le-Ct yereferensi.I-PCDHGB7 gene hypermethylation isimo esihle okanye esibi simiselwa ngokwexabiso likaMe.
Iimeko zesicelo
Ukuhlolwa kwangethuba
Abantu abasempilweni
Uvavanyo loMngcipheko woMhlaza
Amaqela asemngciphekweni omkhulu (abantu abopha ngokungaqhelekanga kwilungu lobufazi emva kokuyeka ukuya exesheni, ukujiya kwe-endometrial, njl.njl.)
Ukubeka iliso kwakhona
Abemi bentelekelelo
Ukubaluleka kweklinikhi
Uvavanyo kwangethuba kubantu abasempilweni:Umhlaza we-Endometrial kunye nezilonda ze-precancerous zingahlolwa ngokuchanekileyo;
Uvavanyo lomngcipheko kuluntu olusemngciphekweni omkhulu:Uvavanyo lomngcipheko lunokwenziwa kubantu abanokopha okungaqhelekanga kwilungu lobufazi kunye nokutyeba kwe-endometrial emva kokuyeka ukuya exesheni ukuncedisa kuxilongo lwezonyango;
Ujongo oluqikelelweyo lokuphindaphinda kwabemi:Ukubeka iliso kokuphindaphinda kwabemi be-Postoperative kunokwenziwa ukuthintela ukulibaziseka kunyango olubangelwa ukuphindaphinda.
Ingqokelela yesampuli
Indlela yesampulu: Beka isampulu yomlomo wesibeleko elahlayo kwi-os yomlomo wesibeleko, gcoba ngobunono ibrashi yomlomo wesibeleko kwaye ujikelezise amaxesha angama-4-5 ngaxeshanye, susa kancinci ibrashi yomlomo wesibeleko, uyibeke kwisisombululo sokugcinwa kweeseli, kwaye uyibhalele kolu vavanyo lulandelayo.
Ukugcinwa kweisampulu:Iisampulu zingagcinwa kwiqondo lobushushu ukuya kutsho kwiintsuku eziyi-14, kwi-2-8 ℃ ukuya kwiinyanga ezi-2, kwaye ku -20±5℃ ukuya kwiinyanga ezingama-24.
Inkqubo yokukhangela: Iiyure ezi-3 (Ngaphandle kwenkqubo yezandla)
I-TAGMe DNA Methylation Detection Kits (qPCR) ye-Endometrial Cancer
| Isicelo seklinikhi | Ukuxilongwa kweklinikhi yokuncedisa i-endometrial carcinoma |
| Ufuzo lokufumanisa | PCDHGB7 |
| Uhlobo lwesampuli | Imizekelo yomlomo wesibeleko yabasetyhini |
| Indlela yokuvavanya | Itekhnoloji yePCR yobungakanani be-Fluorescence |
| Iimodeli ezisebenzayo | ABI7500 |
| Ukupakisha ukucaciswa | Iimvavanyo ezingama-48/ikhithi |
| Iimeko zokuGcina | IKit A kufuneka igcinwe ku-2-30℃ IKit B kufuneka igcinwe ku -20±5℃ Isebenza ukuya kutsho kwiinyanga ezili-12. |
Ngathi
I-Epiprobe inolwakhiwo olubanzi lwezakhiwo: Iziko lokuvelisa i-GMP ligubungela indawo ye-2200 square metres, kwaye igcine inkqubo yokulawula umgangatho we-ISO13485, ehlangabezana neemfuno zemveliso yazo zonke iintlobo zeemveliso zokuvavanya imfuza;Ilebhu yezonyango igubungela indawo ye-5400 square metres kwaye inamandla okwenza ishishini lokufumanisa umhlaza njengelebhu yonyango eqinisekisiweyo yomntu wesithathu.Ngaphandle koko, sineemveliso ezintathu ezifumene isatifikethi se-CE, ezigquma umhlaza womlomo wesibeleko, umhlaza we-endometrial kunye nokufunyaniswa komhlaza we-urothelial.
Itekhnoloji yokufumanisa umhlaza we-Epiprobe ye-molecule ye-molecular ingasetyenziselwa ukuhlolwa komhlaza kwangethuba, ukuxilongwa kwe-axiliary, ukuhlolwa kwangaphambili kunye novavanyo lwasemva kokuhlinzwa, ukujongwa kwe-recrudescence, eqhuba yonke inkqubo yokuxilongwa komhlaza kunye nonyango, ukubonelela ngezisombululo ezingcono koogqirha kunye nezigulane.
