I-TAGMe DNA Methylation Detection Kits (qPCR) ye-Urothelial Cancer
IIMPAWU ZEMVELISO
Ukuchaneka
Ukuqinisekiswa kwiisampuli zeklinikhi ze-3500 kwizifundo zamaziko amaninzi aphindwe kabini, imveliso inomlinganiselo othile we-92.7% kunye novakalelo lwe-82.1%.
Iluncedo
Iteknoloji yokufumanisa i-Me-qPCR ye-methylation yasekuqaleni ingagqitywa kwinqanaba elinye kwiiyure ze-3 ngaphandle kokuguqulwa kwe-bisulfite.
Ayina-invasive
Kuphela yi-30 mL yesampulu yomchamo efunekayo ukuze kufunyanwe iintlobo ezi-3 zomhlaza, kubandakanywa umhlaza wesibeleko sezintso, umhlaza wesinyi, umhlaza wesinyi ngaxeshanye.
Iimeko zesicelo
Uxilongo oluNcedisayo
Abantu abaphethwe yi-hematuria engenabuhlungu/ abarhanelwa ukuba bane-urothelial (umhlaza wesibeleko/ umhlaza wesibeleko sezintso)
Uvavanyo loMngcipheko woMhlaza
Utyando/ichemotherapy-efuna abantu abane-urothelial carcinoma;
Ukubeka iliso kwakhona
Abemi bePostoperative kunye ne-urothelial carcinoma
UKUSETYENZISWA OKUHLOSIWEYO
Le khithi isetyenziselwa ukufumanisa umgangatho we-in vitro we-hypermethylation ye-Urothelial Carcinoma (UC) gene kwi-urothelial specimens.Isiphumo esihle sibonisa ukwanda komngcipheko we-UC, efuna i-cystoscope eyongezelelweyo kunye / okanye uvavanyo lwe-histopathological.Ngokuchasene noko, iziphumo zovavanyo olubi zibonisa ukuba umngcipheko we-UC uphantsi, kodwa umngcipheko awunakukhutshelwa ngaphandle ngokupheleleyo.Ukuxilongwa kokugqibela kufuneka kusekelwe kwi-cystoscope kunye / okanye iziphumo ze-histopathological.
UMGAQO WOKUFUMANA
Le kit iqulethe i-nucleic acid extraction reagent kunye ne-PCR yokufumanisa i-reagent.I-Nucleic acid itsalwa nge-magnetic-bead-based method.Le khithi isekelwe kumgaqo we-fluorescence quantitative PCR indlela, usebenzisa i-methylation-specific real-time PCR reaction ukuhlalutya i-template ye-DNA, kwaye ngaxeshanye ibone iindawo ze-CpG ze-UC gene kunye ne-marker yokulawula umgangatho yangaphakathi ye-reference gene fragments G1 kunye ne-G2.Inqanaba le-methylation ye-UC gene, ebizwa ngokuba yi-Me value, ibalwa ngokwe-UC gene methylated DNA amplification ixabiso le-Ct kunye nexabiso le-Ct yereferensi.I-UC gene hypermethylation isimo esihle okanye esibi sichongwa ngokwexabiso likaMe.
I-DNA Methylation Detection Kits (qPCR) ye-Urothelial Cancer
| Isicelo seklinikhi | Ukuxilongwa kweklinikhi yokuncedisa i-urothelial cnacer;utyando/ikhemotherayi yovavanyo lwempumelelo yonyango;esweni emva kokuhlinzwa kwakhona |
| Ufuzo lokufumanisa | UC |
| Uhlobo lwesampuli | Isampulu yeseli ekhutshiweyo yomchamo (intlenga yomchamo) |
| Indlela yokuvavanya | Itekhnoloji yePCR yobungakanani be-Fluorescence |
| Iimodeli ezisebenzayo | ABI7500 |
| Ukupakisha ukucaciswa | Iimvavanyo ezingama-48/ikhithi |
| Iimeko zokuGcina | IKit A kufuneka igcinwe ku-2-30 ℃ IKit B kufuneka igcinwe ku -20±5℃ Isebenza ukuya kutsho kwiinyanga ezili-12. |
