IKit yokutsalwa kwe-Nucleic Acid (A01)
Umgaqo wokufumanisa
Emva kokukhulula i-DNA ye-genomic ngokwahlula iiseli nge-lysis buffer, i-bead magnetic inokukhetha ngokukhetha i-genomic DNA kwisampulu.Inani elincinane lobumdaka obufunxwa liso lemagnethi linokususwa ngesithinteli sokuhlamba.Kwi-TE, i-bead yamagnetic inokukhulula i-DNA ye-boundgenome, ifumane i-DNA ye-genome ephezulu.Le ndlela ilula kwaye ikhawuleza kwaye umgangatho we-DNA okhutshiweyo uphezulu, onokuhlangabezana nemfuneko yokufunyanwa kwe-DNA methylation.Ngeli xesha, ikhithi yokutsalwa esekwe kwi-bead yamagnetic inokuhambelana ne-automatic nucleic acid extraction, ukuhlangabezana ne-high-throughput nucleic acid extraction tasks.
Amacandelo aphambili e-reagent
Amacandelo aboniswe kwitheyibhile 1:
Itheyibhile 1 Amacandelo e-Reagent kunye nokuLayisha
| Igama lelungu | Amacandelo aphambili | Ubukhulu (48) | Ubungakanani (200) |
| 1. Isisombululo seLysis | Guanidine hydrochloride, Tris | 11 mL / ibhotile | 44 mL / ibhotile |
| 2. Izisombululo zokucoca A | NaCl, Tris | 11 mL / ibhotile | 44 mL / ibhotile |
| 3. Izisombululo zokucoca B | NaCl, Tris | 13 mL / ibhotile | 26.5mL/ibhotile *2 |
| 4. Eluent | Tris, EDTA | 12 mL / ibhotile | 44 mL / ibhotile |
| 5. Isisombululo seProtease K | UProtease K | 1.1mL / iqhekeza | 4.4mL / iqhekeza |
| 6. Ukunqunyanyiswa kwamaso ngemagnethi 1 | Amaso magnetic | 1.1mL / iqhekeza | 4.4mL / iqhekeza |
| 7. Imiyalelo yokukhupha i-nucleic acid reagents |
| Ikopi enye | Ikopi enye |
Izinto ezifunekayo kwi-nucleic acid extraction, kodwa azifakwanga kwikhithi:
1. I-Reagent: I-ethanol e-anhydrous, isopropanol kunye ne-PBS;
2. Izinto ezisetyenziswayo: I-50ml centrifuge tube kunye ne-1.5ml EP tube;
3. Izixhobo: Iketile yokuhlamba amanzi, i-pipettor, i-shelf magnetic, i-centrifuge, i-96-hole deep plate (automatic), i-automatic nucleic acid extraction equipment (automatic).
Ulwazi olusisiseko
Iimfuno zesampulu
1. Ukungcoliswa komnqamlezo phakathi kweesampuli kufuneka kugwenywe ekuqokeleleni isampuli kunye nokugcinwa.
I-2.Ukufumanisa kuya kugqitywa phantsi kokugcinwa kweentsuku ze-7 zokushisa kwe-ambient emva kokuqokelela isampuli yeseli ye-cervical exfoliated (engalungiswanga).Ukufumanisa kuya kugqitywa phantsi kokugcinwa kweentsuku ezingama-30 zobushushu be-ambient emva kokuqokelelwa komzekelo womchamo;Ubhaqo luyakugqitywa ngexesha emva kokuqokelelwa kweesampulu zeeseli ezikhulisiwe.
Iinkcukacha zokupaka:200 iipcs / ibhokisi, 48 pcs / ibhokisi.
Iimeko zokugcina:2-30℃
Ixesha lokuqinisekisa:Iinyanga ezili-12
Isixhobo esisebenzayo:I-Tianlong NP968-C isixhobo se-nucleic acid extraction, i-Tiangen TGuide S96 i-nucleic acid extraction instrument, i-GENE DIAN EB-1000 isixhobo sokukhupha i-nucleic acid.
Isatifikethi serekhodi yesixhobo sonyango Inombolo./imfuno yobugcisa yemveliso Inombolo.: HJXB No. 20210099.
Umhla wokuvunywa kunye nohlengahlengiso lwemiyalelo:
Umhla wokuvunywa: ngoNovemba 18, 2021
