Gargle Nucleic Acid Extraction Reagents
UMGAQO WOKUFUMANA
Imveliso ikakhulu iqulathe gargle, kakhulu-absorbant superparamagnetic nanospheres umxube, kunye unique lysis reagents.Amaso emagnethi adityanisiweyo adibeneyo anobudlelwane obuhle kumalungu omzimba (kubandakanya iintsholongwane ezisimahla kunye neeseli ezosulelwe yintsholongwane).Xa uqhagamshelana nesisombululo se-lysis, i-non-ionic cell / nucleus-membrane-breaking surfactants kunye ne-protease inhibitors kwisisombululo inokuthintela umsebenzi we-DNA / i-RNA enzyme, kwaye izinzile i-nucleic acid.Zonke izinto ze-nucleic acid kumalungu omzimba we-gargle zikhutshwe ngokufanelekileyo kwisisombululo se-lysis, ngokukhawuleza ukufumana i-nucleic acid.Iisampulu ze-gargle zisebenzisa le kiti azifuni ukuhlanjululwa kwe-nucleic acid, enokusebenza ngokuthe ngqo kwikhithi yokufumanisa i-nucleic acid esezantsi.
Amacandelo aphambili e-reagent
Amacandelo aboniswe kwitheyibhile 1.
Itheyibhile 1 Amacandelo kunye nokuLayishwa kwiKit
Igama lelungu | Amacandelo aphambili | Ubungakanani (1) | Ubungakanani (10) | Ubukhulu (30) | Ubungakanani (50) |
1. UGargle A | NaCl | 8mL/ityhubhu | 8mL/ityhubhu *10 iityhubhu | 8mL/ityhubhu *30 iityhubhu | 8mL/ityhubhu *50 iityhubhu |
2. Gargle Collector | PP | Iqhekeza eli-1 | 10 iipcs | 30 iipcs | 50 iipcs |
3. Isisombululo B | Amaso magnetic | 2mL/ityhubhu | 2mL/ityhubhu *10 iityhubhu | 2mL / ityhubhu *30 iityhubhu | 2mL / ityhubhu *50 iityhubhu |
4 ULysis Buffer C | UProtease K | 0.2mL/ iqhekeza | 0.2mL / iqhekeza * 10 iipcs | 0.2mL / iqhekeza * 30 iipcs | 0.2mL / iqhekeza * 50 iipcs |
5. I-Magnetic Cap | Imagnethi | Iqhekeza eli-1 | 10 iipcs | 30 iipcs | 50 iipcs |
Amacandelo asebenza iinyanga ezili-12.
Izinto ezifunekayo kwi-nucleic acid extraction, kodwa azifakwanga kwikhithi:
1. Izinto ezisetyenziswayo: 1.5ml EP tube;
2. Izixhobo: Ukuhlamba amanzi (okanye ukuhlamba isinyithi), iipayipi kunye ne-centrifuge.
Ulwazi olusisiseko
Iimfuno zesampulu:
1. Esebenzayo kwi-nucleic acid extraction kunye nokutyebisa iisampuli ze-gargle.
2. Isampuli ye-gargle iya kongezwa kwisisombululo sokutyebisa B ngexesha emva kokuqokelela.Amaso azibuthe afunyenweyo aya kukhutshelwa kwi-lysis buffer C ngoko nangoko.Iisampulu ezongeziweyo kwi-lysis buffer C zinokugcinwa kwiqondo lobushushu begumbi.
Ukupakishwa kwezinto: 1 isiqwenga / ibhokisi, 10 pcs / ibhokisi, 30 pcs / ibhokisi, kunye 50 pcs / ibhokisi.
Iimeko zokugcina: Isisombululo sokutyebisa B kunye nesisombululo se-lysis kufuneka sigcinwe kwiinyanga ezili-12 kwi-2-8 ℃, kunye namanye amacandelo anokugcinwa kwiinyanga ezili-12 phantsi kobushushu begumbi;Ikhithi inokuthuthwa kwaye igcinwe okwethutyana phantsi kobushushu be-ambient, obungayi kuba ngaphezu kweentsuku ezi-5.
Ixesha lokuqinisekisa: Iinyanga ezili-12
Isatifikethi serekhodi yesixhobo sonyango Inombolo./imfuno yobugcisa yemveliso Inombolo.:HJXB No. 20220086.
Umhla wokuvunywa kunye nohlaziyo lweMiyalelo:
Umhla wemvume: Okt. 26, 2022