I-TAGMe DNA Methylation Detection Kits(qPCR) yoMhlaza woMlomo weSibeleko
IIMPAWU ZEMVELISO
Ukuchaneka
Ukuqinisekiswa kwiisampuli zeklinikhi ze-36000 kwizifundo zamaziko amaninzi aphindwe kabini, imveliso inomlinganiselo othile we-94.3% kunye novakalelo lwe-96.0%.
Iluncedo
Iteknoloji yokufumanisa i-Me-qPCR ye-methylation yasekuqaleni ingagqitywa kwinqanaba elinye kwiiyure ze-3 ngaphandle kokuguqulwa kwe-bisulfite.
Kwangoko
Uvavanyo lomhlaza womlomo wesibeleko lunokuqhubela phambili ukuya kwinqanaba lezilonda ezikwinqanaba eliphezulu (izilonda ezinomhlaza).
Ukuzenzekela
Ikhatshwa yisoftware yohlalutyo lwesiphumo esilungiselelweyo, ukutolika kweziphumo kuzenzekelayo kwaye kufundeka ngokuthe ngqo.
UKUSETYENZISWA OKUHLOSIWEYO
Le khithi isetyenziselwa ukukhangela umgangatho we-in vitro we-hypermethylation ye-PCDHGB7 yemfuza kwimizekelo yomlomo wesibeleko.Isiphumo esihle sibonisa umngcipheko okhulayo webanga le-2 okanye udidi oluphezulu / oluphezulu ngakumbi lwe-cervical intraepithelial neoplasia (CIN2 +, kuquka i-CIN2, i-CIN3, i-adenocarcinoma in situ, kunye nomhlaza womlomo wesibeleko), efuna i-colposcopy eyongezelelweyo kunye / okanye ukuhlolwa kwe-histopathological.Ngokuchasene noko, iziphumo zovavanyo olubi zibonisa ukuba umngcipheko we-CIN2 + uphantsi, kodwa umngcipheko awukwazi ukukhutshwa ngokupheleleyo.Ukuxilongwa kokugqibela kufuneka kusekelwe kwi-colposcopy kunye / okanye iziphumo ze-histopathological.I-PCDHGB7 lilungu losapho lweprotocadherin γ gene cluster.I-Protocadherin ifunyenwe ukulawula iinkqubo zebhayoloji ezifana nokwanda kweeseli, umjikelezo weseli, i-apoptosis, ukuhlasela, ukufuduka kunye ne-autophagy yeeseli ze-tumor ngeendlela ezahlukeneyo zokubonisa, kunye nokuthuliswa kofuzo okubangelwa yi-hypermethylation yommandla womgqugquzeli kuhambelana ngokusondeleyo nokwenzeka kunye nophuhliso. zomhlaza ezininzi.Kuye kwaxelwa ukuba i-hypermethylation ye-PCDHGB7 idibaniswa neentlobo ezahlukeneyo ze-tumor, ezifana ne-non-Hodgkin lymphoma, umdlavuza webele, umhlaza womlomo wesibeleko, umhlaza we-endometrial kunye nomhlaza wesinyi.
UMGAQO WOKUFUMANA
Le kit iqulethe i-nucleic acid extraction reagent kunye ne-PCR yokufumanisa i-reagent.I-Nucleic acid itsalwa nge-magnetic-bead-based method.Le kit isekelwe kumgaqo we-fluorescence quantitative PCR method, usebenzisa i-methylation-specific real-time real-time reaction ye-PCR ukuhlalutya i-template ye-DNA, kwaye ngaxeshanye ibone iziza ze-CpG ze-PCDHGB7 gene kunye ne-marker yokulawula umgangatho yangaphakathi ye-reference gene fragments G1 kunye ne-G2.Inqanaba le-methylation ye-PCDHGB7 kwisampulu, okanye ixabiso le-Me, libalwa ngokwe-PCDHGB7 gene methylated DNA amplification Ct value kunye nexabiso le-Ct yereferensi.I-PCDHGB7 gene hypermethylation isimo esihle okanye esibi simiselwa ngokwexabiso likaMe.
Iimeko zesicelo
Ukuhlolwa kwangethuba
Abantu abasempilweni
Uvavanyo loMngcipheko woMhlaza
Abantu abasemngciphekweni omkhulu (abanomngcipheko omkhulu we-human papillomavirus (hrHPV) okanye i-positive kwi-cytology yokukhutshwa komlomo wesibeleko)
Ukubeka iliso kwakhona
Abemi bePostoperative (enembali yezilonda zomlomo wesibeleko ezikumgangatho ophezulu okanye umhlaza womlomo wesibeleko)
Ukubaluleka kweklinikhi
Uvavanyo kwangethuba kubantu abasempilweni:Umhlaza womlomo wesibeleko kunye nezilonda ezinomhlaza zinokujongwa ngokuchanekileyo
Uvavanyo lomngcipheko kuluntu olusemngciphekweni omkhulu:Ukuhlelwa komngcipheko kunokwenziwa kubantu abane-HPV ukukhokela ukufunyanwa kovavanyo olulandelayo
Ukujongwa kwakhona kwabantu emva kotyando:Ukubeka iliso kokuphindaphinda kwabemi be-Postoperative kunokwenziwa ukuthintela ukulibaziseka kunyango olubangelwa ukuphindaphinda
Ingqokelela yesampuli
Indlela yesampulu: Beka isampulu yomlomo wesibeleko elahlayo kwi-os yomlomo wesibeleko, gcoba ngobunono ibrashi yomlomo wesibeleko kwaye ujikelezise amaxesha angama-4-5 ngaxeshanye, susa kancinci ibrashi yomlomo wesibeleko, uyibeke kwisisombululo sokugcinwa kweeseli, kwaye uyibhalele kolu vavanyo lulandelayo.
Ukugcinwa kweisampulu:Iisampulu zingagcinwa kwiqondo lobushushu ukuya kutsho kwiintsuku eziyi-14, kwi-2-8 ℃ ukuya kwiinyanga ezi-2, kwaye ku -20±5℃ ukuya kwiinyanga ezingama-24.
Inkqubo yokukhangela: Iiyure ezi-3 (Ngaphandle kwenkqubo yezandla)
I-DNA Methylation Detection Kits (qPCR) ye-Urothelial Cancer
| Isicelo seklinikhi | Ukuxilongwa kweklinikhi yokuncedisa umhlaza womlomo wesibeleko |
| Ufuzo lokufumanisa | PCDHGB7 |
| Uhlobo lwesampuli | Imizekelo yomlomo wesibeleko yabasetyhini |
| Indlela yokuvavanya | Itekhnoloji yePCR yobungakanani be-Fluorescence |
| Imodeli esebenzayo | ABI7500 |
| Ukupakisha ukucaciswa | Iimvavanyo ezingama-48/ikhithi |
| Iimeko zokuGcina | IKit A kufuneka igcinwe ku-2-30℃ IKit B kufuneka igcinwe ku -20±5℃ Isebenza ukuya kutsho kwiinyanga ezili-12 |
