I-TAGMe DNA Methylation Detection Kits (qPCR) yoMhlaza wesibeleko / uMhlaza we-Endometrial
IIMPAWU ZEMVELISO
Ayina-invasive
Isebenza ngebrashi yomlomo wesibeleko kunye neesampulu zePap smear.
Iluncedo
Iteknoloji yokufumanisa i-Me-qPCR ye-methylation yasekuqaleni ingagqitywa kwinqanaba elinye kwiiyure ze-3 ngaphandle kokuguqulwa kwe-bisulfite.
Kwangoko
Ifunyaniswe kwinqanaba elingaphambi komhlaza.
Ukuzenzekela
Ikhatshwa yisoftware yohlalutyo lwesiphumo esilungiselelweyo, ukutolika kweziphumo kuzenzekelayo kwaye kufundeka ngokuthe ngqo.
Iimeko zesicelo
Ukuhlolwa kwangethuba
Abantu abasempilweni
Uvavanyo loMngcipheko woMhlaza
Abantu abasemngciphekweni ophezulu (abanomngcipheko ophezulu we-human papillomavirus (hrHPV) okanye i-cytology yokukhutshwa komlomo wesibeleko / i-positive kumngcipheko ophezulu we-papillomavirus yabantu (hrHPV) okanye i-cytology yokukhutshwa komlomo wesibeleko)
Ukubeka iliso kwakhona
Abemi bentelekelelo
UKUSETYENZISWA OKUHLOSIWEYO
Le khithi isetyenziselwa ukukhangela umgangatho we-in vitro we-hypermethylation ye-PCDHGB7 yemfuza kwimizekelo yomlomo wesibeleko.Ngomhlaza womlomo wesibeleko, isiphumo esihle sibonisa umngcipheko onyukileyo webanga lesi-2 okanye ibakala eliphezulu/eliphezulu ngakumbi le-intraepithelial neoplasia yomlomo wesibeleko (CIN2+, kuquka i-CIN2, i-CIN3, i-adenocarcinoma in situ, kunye nomhlaza womlomo wesibeleko), efuna i-colposcopy eyongezelelweyo kunye / okanye ukuhlolwa kwe-histopathological. .Ngokuchasene noko, iziphumo zovavanyo olubi zibonisa ukuba umngcipheko we-CIN2 + uphantsi, kodwa umngcipheko awukwazi ukukhutshwa ngokupheleleyo.Ukuxilongwa kokugqibela kufuneka kusekelwe kwi-colposcopy kunye / okanye iziphumo ze-histopathological.Ngaphezu koko, kumhlaza we-endometrial, isiphumo esihle sibonisa ukwanda komngcipheko wezilonda ze-endometrial precancerous kunye nomhlaza, odinga uvavanyo olongezelelweyo lwe-histopathological ye-endometrium.Ngokuchasene noko, iziphumo zovavanyo olubi zibonisa ukuba umngcipheko wezilonda ze-endometrial precancerous kunye nomhlaza uphantsi, kodwa umngcipheko awunakukhutshelwa ngaphandle ngokupheleleyo.Ukuxilongwa kokugqibela kufuneka kusekelwe kwiziphumo zoviwo lwe-histopathological ye-endometrium.
I-PCDHGB7 lilungu losapho lweprotocadherin γ gene cluster.I-Protocadherin ifunyenwe ukulawula iinkqubo zebhayoloji ezifana nokwanda kweeseli, umjikelezo weseli, i-apoptosis, ukuhlasela, ukufuduka kunye ne-autophagy yeeseli ze-tumor ngeendlela ezahlukeneyo zokubonisa, kunye nokuthuliswa kofuzo okubangelwa yi-hypermethylation yommandla womgqugquzeli kuhambelana ngokusondeleyo nokwenzeka kunye nophuhliso. zomhlaza ezininzi.Kuye kwaxelwa ukuba i-hypermethylation ye-PCDHGB7 idibaniswa neentlobo ezahlukeneyo ze-tumor, ezifana ne-non-Hodgkin lymphoma, umdlavuza webele, umhlaza womlomo wesibeleko, umhlaza we-endometrial kunye nomhlaza wesinyi.
UMGAQO WOKUFUMANA
Le kit iqulethe i-nucleic acid extraction reagent kunye ne-PCR yokufumanisa i-reagent.I-Nucleic acid itsalwa nge-magnetic-bead-based method.Le kit isekelwe kumgaqo we-fluorescence quantitative PCR method, usebenzisa i-methylation-specific real-time real-time reaction ye-PCR ukuhlalutya i-template ye-DNA, kwaye ngaxeshanye ibone iziza ze-CpG ze-PCDHGB7 gene kunye ne-marker yokulawula umgangatho yangaphakathi ye-reference gene fragments G1 kunye ne-G2.Inqanaba le-methylation ye-PCDHGB7 kwisampulu, okanye ixabiso le-Me, libalwa ngokwe-PCDHGB7 gene methylated DNA amplification Ct value kunye nexabiso le-Ct yereferensi.I-PCDHGB7 gene hypermethylation isimo esihle okanye esibi simiselwa ngokwexabiso likaMe.
